Target: HSP40
Conjugate: APC
Product Type: Monoclonal
Clone Number: 3B9.E6
Immunogen: Recombinant Human Protein HSP40 (Hdj1)
Swiss-Prot: P25685
Purification: Protein G Purified
Storage Buffer: PBS pH7.2, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Concentration: 1 mg/ml
Specificity: Detects ~40kDa. Does not cross-react with HDJ2 or YDJ1.
Cellular Localization: Cytoplasm,Nucleus
Scientific Background: Human HSP40/DnaJ proteins comprise a large protein family, members of which feature the J domain (named after the bacterial DnaJ protein) (1). The J-domain spans the first 75 N-terminal amino acids and is separated from the C-terminal by a glycine/phenylalanine-rich domain (2). Members of the HSP40/DnaJ family play diverse roles in many cellular processes, such as folding, translocation, degradation and assembly of multi-protein complexes. In particular, Hdj1, the first human HSP40/DnaJ protein identified, plays an important role in protein translation and folding, as well as in the regulation of HSP70 function (3). HSP40 stimulates the ATPase activity of HSP70 which in turn causes conformational changes of the unfolded proteins (4, 5). The HSP40-HSP70-unfolded protein complex further binds to co-chaperones Hip, Hop and HSP90 which leads to protein folding, or components of protein degradation machinery CHIP and BAG-1 (6). Some studies have shown that the difference between HDJ1 and type 1 DNAJ proteins including HDJ2 and yeast YdjI is the result of the possession of a zinc finger domain by the latter, which helps in the function of protein folding. (7, 8).
References: 1. Cheetham M.E. and Caplan A.J. (1998) Cell Stress Chaperones 3: 28–36.2. Fan C.Y., et al. (2003) Cell Stress Chaperones 8: 309–316.3. Sohn S.Y., Kim S.B., Kim J., and Ahn B.Y. (2006) J Gen Virol. 87(7): 1883-91.4. Liberek K. et al. (1991) Proc. Natl. Acad. Sci. USA 88: 2874–2878.5. Cyr D.M., et al. (1992) J Biol Chem. 267: 20927–20931.6. Höhfeld J., et al. (2001) EMBO Rep. 2: 885–890.7. Terda K., et al. (1997) J Cell Biol. 139: 1089-1095.8. Lu Z. and Cyr D.M. (1998) J Biol Chem. 273: 27824-27830.
Field of Use: Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
English
Deutsch
