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Encompassing Amino Acids: 535-732 (end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: HSP90α (C-terminal):PPID binding activity measured using HSP90α (C-terminal) Inhibitor Screening Assay Kit, BPS Bioscience, Catalog # 50317.
Description: Human Heat Shock Protein 90 alpha (HSP90α), GenBank Accession No. NM_005348, a.a. 535-732 (end) with N-terminal His-tag and N-terminal Avi-tag ™, MW = 25 kDa, expressed in an E. coli expression system and enzymatically biotin-labeled using Avi-tag ™ technology.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl pH 8, 110mM NaCl, 2.2 mM KCl, 0.04% Tween-20,20% glycerol
Genbank: NM_005348
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-tag, N-terminal Avi-tag ™
Uniprot: P07900
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Wayne N. and Bolon D.N. J Biol Chem. 2007 Nov 30;282(48):35386-95.
2. Pearl L.H., Prodromou C. and Workman P. Biochem J. 2008 Mar 15;410(3):439-53.Application Reference(s):
Herbimycins D-F, ansamycin analogues from Streptomyces sp. RM-7-15 (2013)
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