Products from BPS Bioscience require a minimum order value above 400€Encompassing Amino Acids: full length
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Buffer contains 0.1M HEPES, pH 7.4, 0.25M MgCl2, 0.25M KCl, 0.1M Na2MoO4, and 0.05% Triton X. Incubated Hsp90alpha in a series dilution with 5 nM FITC-labeled geldanamycin for 150 minutes at room temperature. Fluorescence polarization measured at ex 470/em528.
Background: Hsp90 (90 kDa heat shock protein) is a molecular chaperone that aids protein folding and quality control for a large number of client proteins. Functional Hsp90 operates as dimer and has intrinsic ATPase activity. The Hsp90 dimer acts in concert with other chaperones (e.g. Hsp70) and is regulated by a number of co-chaperones/accessory proteins (e.g. Hop, cdc37). Hsp90 has been shown to interact with > 100 proteins and some notable clients include kinases (e.g. Raf-1), nuclear hormone receptors (e.g. estrogen receptors), transcription factors (e.g. p53), GPCRs (e.g. CB2 receptors) and ion channels (e.g. CFTR). In humans, the Hsp90beta isoform is constitutively expressed whereas the Hsp90alpha isoforms is expressed under stress conditions. Hsp90 plays an important role in some tumor cell types by stabilising mutated oncogenic proteins.
Description: Human Heat Shock Protein 90α (GenBank Accession No. NM_005348), full length with C-terminal His-tag, MW = 85.5 kDa, expressed in an E. coli expression system.
Format: Aqueous buffer solution
Formulation: 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 225 mM imidazole, 10% glycerol, and 3 mM DTT.
Genbank: NM_005348
Storage Stability: At least 6 months at -80°C.
Tags: C-terminal His-tag
Uniprot: P07900
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Wayne N. and Bolon D.N. J Biol Chem. 2007 Nov 30;282(48):35386-95.
2. Pearl L.H., et al. Biochem J. 2008 Mar 15;410(3):439-53.Application Reference(s):
1. The quinone methide aurin is a heat shock response inducer that causes proteotoxic stress and Noxa-dependent apoptosis in malignant melanoma cells (2015)
2.
Herbimycins D-F, ansamycin analogues from Streptomyces sp. RM-7-15 (2013)
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