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Encompassing Amino Acids: 527-724 (end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: HSP90β (C-terminal):PPID bindingactivity measured using HSP90β (C-terminal)Inhibitor Screening Assay Kit,BPS Bioscience, Catalog # 50314.
Description: Human Heat Shock Protein 90 beta (HSP90β), GenBank Accession No. NM_001271969, a.a. 527-724 (end) with N-terminal His-tag and N-terminal Avi-tag™, MW = 25 kDa, expressed in an E. coli expression system and enzymatically biotin-labeled using Avi-tag™ technology.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl pH 8, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol
Genbank: NM_001271969
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-tag, N-terminal Avi-tag™
Uniprot: P08238
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Milicevic, Z. et al., Int. J. Oncol. 2008Jun;32(6):1169-78.
2. Chao, C.C. et al., J. Cell Biochem. 2008Jul 1;104(4):1286-96.
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