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Encompassing Amino Acids: 1-78
Amino Acid Sequence: MRRGPRSLRG RDAPAPTPCV PAECFDLLVR HCVACGLLRT PRPKPAGASS PAPRTALQPQ ESVGAGAGEA ALPLPGLL
Background: The B cell-activating factor from the TNF family (BAFF), is emerging as an important regulator of B cell and T cell responses. BAFF was originally identified as a factor responsible for B cell survival and maturation .BAFF binds to several receptors. These include transmembrane activator and calcium modulator and cyclophilin ligand interactor (TACI), BAFF-R (BR3), and B cell maturation Ag (BCMA). BAFF-R appears to be particularly important for the regulation of B cell survival and maturation in the spleen, because A/WySnJ mice expressing a defective BAFF-R have disrupted B cell maturation, similar to that seen in BAFF-deficient mice.
Biological Activity: The ED50 , determined by the ability to block BAFF induced survival of splenocyte cells, was found to be in the range of 1.0-5.0 µg/ml.
Description: Recombinant human BAFF-R (B cell-activating factor from the TNF family), also known as CD268, is a disulfide-linked monomeric protein consisting of 76 a.a. and migrates as an approximately 9 kDa protein under reducing and non-reducing conditions. Optimized DNA sequence encoding human BAFF-R extracellular domain was expressed in E. coli.
Endotoxin Level: <0.1 ng/µg (1 EU/µg), using the LAL gel clot method.
Format: lyophilized protein
Formulation: Lyophilized from a 0.2 µm filtered solution containing 2.5% glycine, 0.5% sucrose, 0.01% Tween-80, 5 mM glutamic acid, pH 4.5.
Genbank: Q96RJ3
Purity: ≥95% by SDS-PAGE and HPLC
Reconstitution: Reconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.
Storage Stability: The lyophilized protein is stable for at least 2 years from date of receipt when stored at -20°C. Upon reconstitution, store in working aliquots at +4°C for up to one month, or at -20°C for up to six months, in the presence of a carrier protein. Avoid repeated freeze/thaw cycles.
Uniprot: Q96RJ3
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. The effects of BAFF and BAFF-R-fc fusion protein in immune thrombocytopenia. Blood, Dec 2009, 114: 5362 - 5367.
2. c-rel in the regulation of BAFF-R expression in malignant human B cells. Blood (ASH Annual Meeting Abstracts), Nov 2009, 114: 2400.
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