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Encompassing Amino Acids: 23-109
Amino Acid Sequence: VLEVYYTS LRCRCVQESS VFIPRRFIDR IQILPRGNGC PRKEIIVWKK NKSIVCVDPQ AEWIQRMMEV LRKRSSSTLP VPVFKRKIP
Background: The homeostatic chemokine CXCL13 (also called B cell- attracting chemokine 1 [BCA- 1] or B-lymphocyte chemoattractant [BLC]) is constitutively expressed in secondary lymphoid tissue and initiates lymphoid neogenesis when expressed aberrantly in mice. CXCL13 has also been detected in chronic inflammation associated with human lymphoid neogenesis, suggesting a pathogenic role. Follicular dendritic cells (FDCs) are generally considered to be the major source of CXCL13 both in normal and aberrant lymphoid tissue. CXCL13 has been the exclusive ligand for CXCR5, which initiates chemotaxis toward an increasing gradient in vitro and to B cell zones in lymph nodes that express CXCL13. More recently, CXCR5 expression has also been shown in DN T cells in mice, a subset of CD4 follicular Th cells, and some tonsillar CD4+CD25+ Treg cells in humans.
Biological Activity: Determined by its ability to chemoattract human T cells using a concentration range of 2.0-10.0 ng/ml.
Description: Recombinant human BCA1 (B cell- attracting chemokine 1), also known as CXCL13 or BLC, is a disulfide-linked monomeric protein consisting of 88 a.a. and migrates as an approximately 10 kDa protein under reducing and non-reducing conditions. Human CXCL13, generated by the proteolytic removal of the signal peptide and propeptide, has a calculated molecular mass of ~10 kDa. Optimized DNA sequence encoding human BCA1 mature domain was expressed in E. coli.
Endotoxin Level: <1 EU/µg
Format: lyophilized protein
Formulation: Lyophilized from a 0.2 µm filtered solution containing 20 mM phosphate buffer, pH 7.5, 100 mM NaCl.
Genbank: Q53X90
Purity: ≥98% by SDS-PAGE and HPLC
Reconstitution: Reconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.
Storage Stability: The lyophilized protein is stable for at least 2 years from date of receipt when stored at -20°C. Upon reconstitution, store in working aliquots at +4°C for up to one month, or at -20°C for up to six months, in the presence of a carrier protein. Avoid repeated freeze/thaw cycles.
Uniprot: Q53X90
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Increased cerebrospinal fluid concentrations of the chemokine CXCL13 in active MS. Neurology, Dec 2009, 73: 2003 - 2010.
2. B-cell-attracting chemokine CXCL13 as a marker of disease activity and renal involvement in systemic lupus erythematosus (sle). Nephrol. Dial. Transplant., Dec 2009, 24: 3708 - 3712.
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