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Amino Acid Sequence: VVIP SPCCMFFVSK RIPENRVVSY QLSSRSTCLK AGVIFTTKKG QQFCGDPKQE WVQRYMKNLD AKQKKASPRA RAVA
Background: Eotaxin belongs to the platelet factor-4 family of chemokines. The factor is being referred to also as Eotaxin-2 and has been renamed CCL24. Both eotaxin-2/CCL24 and eotaxin-3/CCL26 are CC chemokines and functional ligands for CC chemokine receptor 3 (CCR3), which is preferentially expressed on eosinophils. Only 34% of the amino acids in eotaxin-2/CCL24 and eotaxin-3/CCL26 are identical, but these two chemokines have a common characteristic feature in terms of chemotaxis for eosinophils via the CCR3.
Biological Activity: Biological activity was determined by the ability to induce chemotaxis of purified eosinophils at concentrations ranging between 50-100 ng/ml.
Description: Recombinant Eotaxin-2 is a disulfide-linked homodimeric protein consisting of 79 amino acid residues, and migrates as an approximately 9 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding human Eotaxin-2 mature chain was expressed in E. coli.
Endotoxin Level: <0.1 ng/µg (1 EU/µg), using the LAL gel clot method.
Format: lyophilized protein
Formulation: Lyophilized from 0.2 µm filtered concentrated (1.0 mg/ml) solution in 100 mM NaCl, 10 mM phosphate buffer, pH 7.0.
Genbank: O00175
Purity: ≥97% by SDS-PAGE and HPLC
Reconstitution: Reconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.
Storage Stability: The lyophilized protein is stable for at least 2 years from date of receipt when stored at -20°C. Upon reconstitution, store in working aliquots at +4°C for up to one month, or at -20°C for up to six months, in the presence of a carrier protein. Avoid repeated freeze/thaw cycles.
Uniprot: O00175
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Clin. Cancer Res., Oct 2007, 13: 5719 - 5728.
2. J. Biol. Chem., Apr 2005, 280: 13952 - 13961.
3. J. Immunol., Sep 2002, 169: 2712 - 2718.
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