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Encompassing Amino Acids: 24-94
Amino Acid Sequence: TRGSDIS KTCCFQYSHK PLPWTWVRSY EFTSNSCSQR AVIFTTKRGK KVCTHPRKKW VQKYISLLKT PKQL
Background: Eotaxin belongs to the platelet factor-4 family of chemokines. The factor is being referred to also as Eotaxin-2 and has been renamed CCL24. Both eotaxin-2/CCL24 and eotaxin-3/CCL26 are CC chemokines and functional ligands for CC chemokine receptor 3 (CCR3), which is preferentially expressed on eosinophils. Only 34% of the amino acids in eotaxin-2/CCL24 and eotaxin-3/CCL26 are identical, but these two chemokines have a common characteristic feature in terms of chemotaxis for eosinophils via the CCR3.
Biological Activity: Biological activity was determined by the ability to induce chemotaxis of purified eosinophils at concentrations ranging between 50-100 ng/ml.
Description: Recombinant Eotaxin-3 is a disulfide-linked homodimeric protein consisting of 72 amino acid residues, and migrates as an approximately 9 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding human Eotaxin-3 mature chain was expressed in E. coli.
Endotoxin Level: <0.1 ng/µg (1 EU/µg), using the LAL gel clot method.
Format: lyophilized protein
Formulation: Lyophilized from 0.2 µm filtered PBS, pH 7.5 with 10% glycerol.
Genbank: Q9Y258
Purity: ≥97% by SDS-PAGE and HPLC
Reconstitution: Reconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.
Storage Stability: The lyophilized protein is stable for at least 2 years from date of receipt when stored at -20°C. Upon reconstitution, store in working aliquots at +4°C for up to one month, or at -20°C for up to six months, in the presence of a carrier protein. Avoid repeated freeze/thaw cycles.
Uniprot: Q9Y258
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. J Rheumatol, Aug 2009, 36: 1590 - 1595.
2. Rheumatology, Jun 2008, 47: 804 - 808.
3. Eur. Respir. J., Nov 2005, 26: 795 - 803.
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