Human IgG Colorimetric ELISA Kit

Human IgG Colorimetric ELISA Kit
SKU
BPS82612
Packaging Unit
96 reactions
Manufacturer
BPS Bioscience

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Products from BPS Bioscience require a minimum order value above 400€

Application: Quantify human IgG in cell culture supernatants.

Background: IgG, or immunoglobulin G, is one of the most abundant glycoproteins present in human serum. Humans have four different IgG subclasses, IgG1-4, where the number corresponds to their abundance. IgG are involved in complement activation, phagocytosis, and toxin neutralization. Each subclass binds to different FcγR receptors, with varying affinities. Out of the four isotypes, IgG1 accounts for 65% of all IgG and has high affinity for most of the FcγRs, but its affinity for FcRn (neonatal Fc receptor for IgG) is pH dependent (higher at pH 6.5 or below). The binding of FcRn can be exploited by engineering therapeutic antibodies to increase their binding to FcRn, thereby improving their half-life and therapeutic efficacy. For example, an antibody cocktail that contains Fc mutations, and thus an extended half-life (Evusheld) has been used to treat COVID-19. There are now several other drugs in clinical using similar strategies. Further studies and drug development taking advantage of FcRn properties will provide new therapeutic options. The ability to accurately detect human IgG levels with an easy readout is thus a necessary tool to advance the development of such therapeutic tools.

Contraindications: The IgG Colorimetric ELISA Kit is compatible with up to 1% final DMSO concentration.

Description: The IgG Colorimetric ELISA Kit is designed to detect and quantify protein levels of human IgG captured by pre-matched antibody pairs. This assay kit comes in a convenient 96-well format, with a pre-coated IgG-specific antibody plate, enough recombinant purified IgG1 standard, and detection reagents for 100 wells.

Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed

Warnings: Avoid freeze/thaw cycles

Biosafety Level: Not applicable (BSL-1)

References: Vidarsoon G, et al., 2014 Front. Immunol. 5:00520
More Information
SKU BPS82612
Manufacturer BPS Bioscience
Manufacturer SKU 82612
Package Unit 96 reactions
Quantity Unit PAK
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