Application Note: Human IgM ELISA kit is designed for the quantitative detection of human IgM in serum, plasma, and hybridoma cell supernatants.
ELISA Dilution: 0.23 ng/ml-15.0 ng/ml
Sensitivity: <250 pg/ml
Detection Range: 0.23 ng/ml - 15.0 ng/ml
General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Purity and Specificity: Rockland’s Human IgM AccuSignal™ ELISA Kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative detection of human IgM in serum, plasma, and cell culture supernatants. This kit contains: Human IgM control, Capture antibody, biotinylated Detection Antibody, streptavidin-peroxidase conjugate, along with buffers and protocol. See protocol for details.
Background: IgM (immunoglobulin M) is the is the third most abundant and largest antibody in the human circulatory system. IgM is expressed on the surface of immature and mature B cells as a monomer and is expressed in a secreted form as a pentamer with very high avidity. IgM is the first human immunoglobulin to appear in response to initial exposure to an antigen and eliminates pathogens in the early stages of B cell-mediated (humoral) immunity before there is sufficient IgG. IgM is a strong complement activator and agglutinator due to its pentameric structure and binds fragment crystallization (Fc) receptors. IgM has a molecular mass of approximately 970 kDa in its pentamer form with ten binding sites. Typically, however, IgM cannot bind 10 antigens at the same time because the large size of most antigens hampers binding to neighboring sites. IgM antibodies do not pass across the human placenta. IgM antibodies are very useful in the diagnosis of infectious diseases. The presence of IgM antibodies in a patient’s serum indicates recent infection, or in a neonate’s serum specifies an intrauterine infection (e.g. congenital rubella).
Low Endotoxin: No
Secondary Antibody Specificity: IgM (H&L)
Other: User Optimized
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