Human IgM Fab mu

Human IgM Fab mu
SKU
ROC009-0109
Packaging Unit
2 mg
Manufacturer
Rockland

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Application Note: Monovalent Fab fragments of affinity-purified, secondary antibodies are offered to cover (block) the surface of immunoglobulins for double labeling primary antibodies from the same host species, or to block endogenous immunoglobulins in tissue sections or on cell surfaces. They can be used for these purposes because each Fab fragment has only a single antigen binding site. HUMAN IgM (myeloma) Fab µ fragment has been tested in SDS-PAge and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments. Specific conditions should be optimized by the user.

Concentration Value: 2.0 mg/mL

ELISA Dilution: User Optimized

Western Blot Dilution: User Optimized

General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

Physical State: Liquid (sterile filtered)

Purity and Specificity: HUMAN IgM (myeloma) Fab µ fragment was prepared from serum by a multi-step process which includes delipidation, selective precipitation, tandem molecular sieve chromatography and trypsin digestion followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Human Serum.    No reaction was observed against anti-Human IgG F(c).  Some light chain cross reactivity will occur with anti-Human IgG.

Background: Human IgM Fab mu is ideal for investigators involved in serum protein component research. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to antigen. The spleen is the major site of specific IgM production. Distinct heavy chains differ in size and composition; alpha and gamma contain approximately 450 amino acids, while μ and ε have approximately 550 amino acids.

Low Endotoxin: No

Other: User Optimized
More Information
SKU ROC009-0109
Manufacturer Rockland
Manufacturer SKU 009-0109
Package Unit 2 mg
Quantity Unit STK
Application SDS-PAGE
Isotype IgM
Conjugate Unconjugated
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