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Encompassing Amino Acids: 37-193
Amino Acid Sequence: YFGK LESKLSVIRN LNDQVLFIDQ GNRPLFEDMT DSDCRDNAPR TIFIISMYKD SQPRGMAVTI SVKCEKISTL SCENKIISFKEMNPPDNIKD TKSDIIFFQR SVPGHDNKMQ FESSSYEGYF LACEKERDLF KLILKKEDELGDRSIMFTVQ NED
Background: IL-18 is synthesized as a biologically inactive precursor protein that has limited biological activity. The proform of IL18 is processed by one of the caspases, Caspase-1, to generate the 18 kDa bioactive molecule. IL-18 is produced during the acute immune response by macrophages and immature dendritic cells. Interleukin-18 is expressed by a variety of immune and non-immune cells, including monocytes and macrophages. IL-18 is one of the pro-inflammatory cytokines. The activities of IL-18 appear to be species-specific. An important function of IL-18 is the regulation of functionally distinct subsets of T-helper cells required for cell mediated immune responses. IL-18 functions as a growth and differentiation factor for Th1 cells. IL-18 up-regulates FAS ligand mediated cytotoxic activitiy of murine natural killer cells. IL-18 is part of a complex regulatory circuit involved in causing cell death by apoptosis.
Biological Activity: The ED50 was determined by the dose-dependent proliferation of human PBMC co-stimulated with IL-12 cells was found to be in the range of 5 ng/ml.
Description: Recombinant Interleukin-18 is a disulfide-linked monomer protein consisting of 158 amino acid residues, migrates as an approximately 18 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding human Interleukin-18 mature chain was expressed in E. coli.
Endotoxin Level: <0.1 ng/µg (1 EU/µg), using the LAL gel clot method.
Format: lyophilized protein
Formulation: Lyophilized from 0.2 µm filtered PBS, pH 7.
Genbank: Q14116
Purity: ≥98% by SDS-PAGE and HPLC
Reconstitution: Reconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.
Storage Stability: The lyophilized protein is stable for at least 2 years from date of receipt when stored at -20°C. Upon reconstitution, store in working aliquots at +4°C for up to one month, or at -20°C for up to six months, in the presence of a carrier protein. Avoid repeated freeze/thaw cycles.
Uniprot: Q14116
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Tsutsui H, Nakanishi K. Immunotherapy. 2012 Dec,4(12):1883-94.
2. Barker BR, et al. Curr Opin Immunol. 2011 Oct,23(5):591-7
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