Species: Human
Application Note: 1. Measured by its binding ability in a functional ELISA. Immobilized recombinant Human PD1-His protein at 10 μg/ml (100 μl/well) can bind Human PD-L1 protein (active) (GTX01279-pro) with a linear range of 1.28-20 μg/ml.
2. Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion in human T cell lymphocytes. The ED₅₀ for this effect is typically 0.4-3 μg/ml.
Form: Lyophilized powder
Buffer (with preservative): Batch dependent (please contact us for details)
Concentration:
Background: This gene encodes an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
Uniprot ID: Q9NZQ7
Region Sequence: C-terminal mouse IgG1-Fc tagged; Met1-Thr239 of Human PD-L1 protein (NP_054862.1)
ExpressionSystem: HEK293 cells
Conjugation: Unconjugated
Full Name: CD274 molecule
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