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Application: Screen small molecule inhibitors in high throughput screening (HTS) applications.
Background: IDE (Insulin-degrading enzyme) is a zinc metallopeptidase with a ubiquitous pattern of expression in humans. Although initially identified as an insulin degrading protein, it can also interact with glucagon, somatostatin, β-amyloid and natriuretic peptide. IDE is involved in multiple pathways, such as Varicella Zoster Virus infection, steroid receptor signaling and proteasomal modulation of insulin. More recently IDE was identified as a heat-shock like protein capable of processing antigenic peptides presented by MHC class I. Dysregulation of IDE may be involved in Alzheimer's disease, Type 2 Diabetes Mellitus, and it may play a role in neuroblastoma growth. The identification of IDE inhibitors is crucial for the understanding of IDE role in disease and potential therapeutical approaches.
Description: The IDE (Insulin-degrading enzyme) Inhibitor Screening Assay is a homogeneous fluorogenic assay designed to measure the activity of IDE for screening and profiling applications. The IDE assay kit comes in a convenient 96-well or 384-well format and contains enough purified IDE (amino acids 71-254), substrate, and PR-02 buffer for 100 or 400 reactions.
Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Uniprot: P14735
Warnings: Avoid freeze/thaw cycles
Biosafety Level: Not applicable (BSL-1)
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