Clone Name: R132H
Light Chain: Kappa
Application Note: IHC-P: 1:25-1:50. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Calculated MW: 47
Positive Control: Human Glioma tissue
Specificity/Sensitivity: This antibody is specific for R132H mutation of human IDH1 protein and does not cross react with other mutations.
Form: Liquid
Buffer (with preservative): Tissue culture supernatant, 0.09% Sodium azide.
Concentration:
Background: Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Sep 2013]
Uniprot ID: O75874
Antigen Species: Human
Immunogen: KLH-conjugated linear peptide corresponding to human Isocitrate Dehydrogenase 1 (IDH1)
Purification: Unpurified
Conjugation: Unconjugated
Full Name: isocitrate dehydrogenase (NADP(+)) 1, cytosolic
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