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Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: IDH reductive activity was measured in 200 µl reaction containing 25 mM Tris (pH 7.4), 150 mM NaCl, 10 mM MgCl2, 0.03% BSA, 1 mM alpha-Ketoglutarate, 10 µM NADPH and IDH. Depletion of NADPH was monitored continuously at Abs340 nm for 20 min. Molar extinction coefficient of NADPH is 6,200 M-1cm-1.
Background: Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). The IDH2 homodimer is an the NADP(+)-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production and may tightly associate or interact with the pyruvate dehydrogenase complex.
Description: Human isocitrate dehydrogenase 2, also known as IDH2, GenBank Accession No. NM_002168, a.a. 1-452(end) with with Arg to Gln mutation on a.a. 140 and C-terminal FLAG-tag, MW = 52 kDa, expressed in a baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 80 µg/ml FLAG peptide, 0.04% Tween-20, and 20% glycerol.
Genbank: NM_002168
Storage Stability: At least 6 months at -80°C.
Tags: C-terminal FLAG-tag
Uniprot: P48735
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Schaap, F.G., et al., Adv. Anat. Pathol. 2013;20(1):32-38.
2. Grassian, A.R., et al., J. Biol. Chem. 2012;287(50):42180-42194.
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