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Applications: Monitor IDO1 pathway activity. Screen for activators or inhibitors of IDO1 in a cellular context
Background: L-tryptophan (L-Trp) is an essential amino acid necessary for protein synthesis inmammalian cells and the L-Trp to kynurenine (Kyn) pathway is firmly established as a keyregulator of innate and adaptive immunity. Catabolism of L-Trp to Kyn maintains animmunosuppressive microenvironment by starving immune cells of L-Trp and releasingdegradation products of L-Trp that have immunosuppressive functions. Indoleamine 2,3-dioxygenases (IDO1 & IDO2), two of the rate limiting enzymes in this pathway, are upregulatedin many tumors, providing cancer cells with an avenue for immune evasion.
Description: Stable Recombinant HEK293 cell line expressing tetracycline-inducible human indoleamine 2,3-dioxygenase (IDO1) Genbank accession number NM_002164.
Format: Aqueous solution containing DMSO
Genbank: NM_002164
Host Cell Line: HEK293
Mycoplasma Testing: The cell line has been screened using the PCR-based Venor™GeM Mycoplasma Detection kit(Sigma-Aldrich) to confirm the absence of Mycoplasma species.
Storage Stability: Immediately upon receipt, store vials in liquid nitrogen.
Supplied As: Each vial contains ~1.5 X 106 cells in 1 ml of 10% DMSO
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: BSL-2
References: 1. Yue, E., et al., J. Med. Chem. 2009; 52: 7364-7367.
2. Liu, X., et al., Blood. 2010; 115(17): 3520-3530.
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