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Encompassing Amino Acids: 2-407
Applications: Useful for studying enzyme kinetics, substrate specificity, and screening inhibitors.
Assay Conditions: Assay was performed in20 mM ascorbate, 10 mM methylene blue,10 µg/ml catalase, 100 µM NaPi, pH 6.5containing varying concentrations oftryptophan. 300 ng of IDO1 was added toinitiate reaction and aliquots were collectedat various time points for Kyn analysis.
Description: Mouse IDO1, also known as Indoleamine 2,3-dioxygenase 1, GenBank Accession No. NM_008324, a.a. 2-407(end) with an Nterminal His-tag, expressed in the presence of hemin in an E. coli cell expression system. MW = 46 kDa.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol
Genbank: NM_008324
Storage Stability: At least 6 months at -80°C (for all proteins)
Tags: Nterminal His-tag
Uniprot: P28776
Warnings: Avoid freeze/thaw cycles. Storing diluted protein is not recommended. If necessary, use carrier protein (BSA 0.1 - 0.5%).
Biosafety Level: Not applicable (BSL-1)
References: 1. Lob, S. et al., Cancer Immunol. Immunother. 2009; 58(1): 153-157.
2. Liu, X. et al., Blood. 2010; 115(17): 3520-3530.
3. Flick, H.E., et al., Int. Nat. J. Tryptophan Res. 2013; 6: 35-45.
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