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Application: Monitor IDO2 pathway activity. Screen for activators or inhibitors of IDO2 in a cellular context.
Background: L-Trp is an essential amino acid necessary for protein synthesis in mammalian cells, and the L-Trp to Kyn pathway is firmly established as a key regulator of innate and adaptive immunity. Catabolism of L-Trp to Kyn maintains an immunosuppressive microenvironment by starving immune cells of L-Trp and releasing degradation products of L -Trp that have immunosuppressive functions. IDO2 is upregulated in many tumors, providing cancer cells with an avenue for immune evasion.
Description: The IDO2 Cellular Activity QuickDetect™ Supplements are designed to complement the hIDO2-HEK293 Recombinant Cell Line (BPS Cat. #60532) or other IDO2-expressing cell lines. This kit contains the cell culture medium supplements necessary for activation of IDO2 and for the analysis and detection of indoleamine 2,3 dioxygenase (IDO2)-catalyzed conversion of L-tryptophan (L-Trp) to Kynurenine (Kyn). The supplements and the detection reagents, when used as described, allow for indirect measurement of Kyn levels by analyzing absorption at 480 nm.
Storage Stability: Stable for at least 6 months when stored as directed.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1.Metz, R., et al., Int. Immunol. 2014; 26: 35-367. 2.Fatokun, A., et al., Amino Acids 2013; 45: 1319-1329.
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