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Encompassing Amino Acids: 2-407(end)
Applications: Useful for studying enzyme kinetics, substrate specificity, and screening inhibitors.
Assay Conditions: Assay was performedfollowing the protocol in BPS Cat. #72022using varying concentrations of IDO2.
Description: Mouse IDO2, also known as Indoleamine 2,3-dioxygenase 2, GenBank Accession No. NM_145949, a.a. 2-407(end) with an N-terminal His-tag, expressed in the presence of hemin in an E. coli cell expression system. MW = 46 kDa.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol
Genbank: NM_145949
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-tag
Uniprot: Q8R0V5
Warnings: Avoid freeze/thaw cycles. Storing diluted protein is not recommended. If necessary, use carrier protein (BSA 0.1 - 0.5%).
Biosafety Level: Not applicable (BSL-1)
References: 1. Metz, R., et al., Canc. Res. 2007;67(15): 7082-7087.
2. Witkiewicz, A.K., et al., J. Am. Coll.Surgeons. 2009; 208(5): 781-787.
3. Flick, H.E., et al., Int. Nat. J. TryptophanRes. 2013; 6: 35-45.
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