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Encompassing Amino Acids: 15-420(end)
Applications: Useful for studying enzyme kinetics, substrate specificity, and screening inhibitors.
Assay Conditions: IDO2 activity was confirmed using the IDO2 Inhibitor Screening Assay Kit (BPS Cat. #72022) and following the protocol provided.
Description: Human IDO2, also known as Indoleamine 2,3-dioxygenase 2, GenBank Accession No. NM_194294, a.a. 15-420(end) with an N-terminal His-tag, expressed in the presence of hemin in an E. coli cell expression system. MW = 46 kDa.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0,110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, 3 mM DTT and 150 mMimidazole.
Genbank: NM_194294
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-tag
Uniprot: Q6ZQW0
Warnings: Avoidfreeze/thaw cycles. Storing diluted enzymeis not recommended, if necessary, usecarrier protein (BSA 0.1 - 0.5%).
Biosafety Level: Not applicable (BSL-1)
References: 1. Metz, R., et al., Canc. Res. 2007; 67(15): 7082-7087.
2. Witkiewicz, A.K., et al., J. Am. Coll. Surgeons. 2009; 208(5): 781-787.
3. Flick, H.E., et al., Int. Nat. J. Tryptophan Res. 2013; 6: 35-45.
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