Specificity: Human 15-lipoxygenase-2 (15-LO-2) amino acids 161-179 (CLDEKTVEDLELNIKYSTA. Antibody did not cross-react with 15-LO-1 in Western testing using recombinant protein.
Antibody Modification: Unconjugated
Antigen Modification: aa161-179
Presentation: TBS, pH 7.4, 50% glycerol, 0.1% BSA, 0.02 % sodium azide.
Immunogen: Human 15-lipoxygenase-2 (15-LO-2) amino acids 161-179 (CLDEKTVEDLELNIKYSTA). Percent identity by BLAST analysis: Human (100%); Gorilla (94%).
Gene: ALOX15B / 15-LOX-2
Description: 15-LOX-2 antibody LS-B1588 is an unconjugated rabbit polyclonal antibody to human 15-LOX-2 (ALOX15B) (aa161-179). Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Usage: Immunohistochemistry: LS-B1588 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1588 was determined to be 5 ug/ml.
Synonyms: ALOX15B, 15S-lipoxygenase, 15-lipoxygenase 2, 15-LOX-2, 15-LOX-B, Arachidonate 15-lipoxygenase B, Arachidonate 15-lipoxygenase 2
Concentration: 0.4 mg/ml
Recommended Storage: Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
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