Clone: 1D4
Specificity: GAPDH (1D4).
Antibody Modification: Unconjugated
Presentation: 50% PBS, 50% Glycerol, 5mm sodium azide.
Immunogen: The immunogen used to raise this particular antibody was extensively purified pig GAPDH.
Gene: GAPDH
Description: GAPDH antibody LS-B83 is an unconjugated mouse monoclonal antibody to GAPDH from pig. It is reactive with human, mouse, rat and other species. Validated for IF, IHC, IP and WB.
Usage: Immunohistochemistry: LS-B83 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B83 was determined to be 1.25-2.5 ug/ml.
Synonyms: GAPDH, A1 40 kd subunit, Activator 1 40 kd subunit, G3PD, GAPD, G3pdh, Rfc40, Rf-c 40 kd subunit
Concentration: 1 mg/ml
Recommended Storage: Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
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