Specificity: Human MRGPRX2 / MRGX2. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except MRGPRX1 (56%), MRGPRX3 (50%).
Antibody Modification: Unconjugated
Antigen Modification: Extracellular Domain
Presentation: PBS, 0.1% Sodium Azide
Immunogen: Synthetic 16 amino acid peptide from 3rd extracellular domain of human MRGPRX2 / MRGX2. Percent identity with other species by BLAST analysis: Human (100%); Chimpanzee, Gorilla, Monkey (94%); Orangutan (88%); Gibbon (81%).
Gene: MRGPRX2 / MRGX2
Description: MRGX2 antibody LS-A6645 is an unconjugated rabbit polyclonal antibody to human MRGX2 (MRGPRX2) (Extracellular Domain). Validated for IHC. Tested on 20 paraffin-embedded human tissues.
Usage: Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 20 ug/ml.
Synonyms: MRGPRX2, MRGX2, MAS-related GPR, member X2
Recommended Storage: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Concentration: 1 mg/ml
English
Deutsch
