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Encompassing Amino Acids: 20-155(end)
Application: Useful for studying protein function and protein-protein interaction. studies.
Assay Conditions: 100 ng of IL-17A wascoated onto a 96-well plate overnight. Theindicated amount of IL-17RA (BPS Cat.#91013), Biotin was added in IL-17 AssayBuffer and allowed to incubate at roomtemperature for 2 hr. Strep-HRP was added,followed by chemiluminescent substrate anddetection.
Description: Human secreted interleukin17 A (IL-17A) also known as, IL-17 andcytotoxic T lymphocyte antigen 8 (CTLA8)GenBank Accession No. NM_ 002190, a.a20-155(end), expressed in an e. coliexpression system. This protein existsnaturally as a homodimer; M.W. = 17.5 kDa(monomer); 35 kDa dimer
Format: Aqueous buffer solution
Formulation: 10 mM sodium phosphate, pH 6.0, and 220 mM NaCl
Genbank: NM_ 002190
Storage Stability: At least 6 months at -80°C.
Uniprot: Q16552
Warnings: Avoid freeze/thaw cycles. Protein may be diluted to ≥ 100 g/ml in PBS + glycerol and stored at -80°C.
Biosafety Level: Not applicable (BSL-1)
References: 1. Iwakura Y, et al. Immunol Rev. 2008. 226:57-79.
2. Bullens DM, et al. Clin Dev Immunol. 2013. 2013:840315.
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