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Encompassing Amino Acids: 33-320
Applications: Useful for studying protein binding and screening small molecules for drug discovery.
Assay Conditions: IL-17RA-IL-17A[B] binding activity was measured using the IL-17RA:IL-17A[Biotin] Binding Assay Kit, BPS Bioscience #79891 and Anti-IL-17A Antibody, BPS Bioscience #91015.
Description: Human Interleukin-17A (IL-17A), also known as CD217 and CANDF5, GenBank Accession No. NM_ 014339, a.a 33-320, fused at the C-terminus of the Fc portion of human IgG1, with C-terminal Avi-tag™, expressed in a HEK293 cell expression system, MW=62 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Format: Aqueous buffer solution
Formulation: 8 mM Phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.
Genbank: NM_ 014339
Host Cell Line: HEK293
Storage Stability: At least 6 months at -80°C.
Supplied As: Aqueous buffer solution.
Tags: C-terminal Fc-Avi-tag
Uniprot: Q96F46
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Wright, J.F., et. al. J. Immun. 2008; 181(4): 2799-2805.
2. Rickel, E.A., et al., J. Immun. 2008; 181(6): 4299-4310.
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