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Encompassing Amino Acids: 33-320
Application: Useful for studying protein function and protein-protein interaction.
Assay Conditions: 100 ng of IL-17A (BPSCat. #91014) was coated onto a 96-wellplate overnight. The indicated amount of IL-17RA, Biotin was added in IL-17 AssayBuffer and allowed to incubate at roomtemperature for 2 hr. Strep-HRP was added,followed by chemiluminescent substrate anddetection.
Description: Human secreted interleukin17 receptor A (IL-17RA) Fc fusion protein,also known as, IL-17 receptor A andCD217, GenBank Accession No. NM_014339, a.a 33-320, fused at the C-terminusto the Fc portion of human IgG1 with a CterminalAviTag™, expressed in a HEK293cell expression system. The protein isenzymatically biotinylated using AviTag™technology. This protein runs at a higherM.W. by SDS-PAGE due to glycosylation.
Format: Aqueous buffer solution
Formulation: 8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, pH 7.4, and 20% glycerol
Genbank: NM_ 014339
Host Cell Line: HEK293
Storage Stability: At least 6 months at -80°C.
Tags: Fc Fusion (Human IgG1)
Uniprot: Q96F46
Warnings: Avoid freeze/thaw cycles. Protein may be diluted to ≥ 100 g/ml in PBS + glycerol and stored at -80°C.
Biosafety Level: Not applicable (BSL-1)
References: 1. Iwakura Y, et al. Immunol Rev. 2008. 226:57-79.
2. Bullens DM, et al. Clin Dev Immunol. 2013. 2013:840315.
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