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Application: This kit is useful for cytokine detection in cell culture medium.
Description: The cytokine protein Interleukin-1β is a mediator in the inflammatory response and is a therapeutic target for autoinflammatory diseases such as Alzheimer's disease and Schnitzler syndrome. The Colorimetric Human IL-1β Detection Kit is designed for detecting and quantifying human interleukin-1β in cell culture medium. Only a few simple steps on a microtiter plate are required for the assay. First, the capture antibody is coated on a 96-well plate. Next, samples containing IL-1β are incubated on the coated plate followed by detecting the captured IL-1 β with the detection antibody. Finally, the plate is treated with streptavidin-HRP followed by addition of a colorimetric HRP substrate to produce color, which can then be measured using a UV/Vis spectrophotometer microplate reader.
Storage Stability: One year from date of receipt when stored as directed.
Supplied As: This kit comes in a convenient 96-well format, with capture and detection antibodies for IL-1β, streptavidin-labeled HRP, blocking buffer, IL-1β standard, and colorimetric HRP substrate for 96-well plates.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Sciaccaa, FL., et al. Interleukin-1B polymorphism is associated with age at onset of
Alzheimer's disease. Neurobiology of Aging 2003. 24: 927-931.
2. Roerink, M., et al. Interleukin-1 as a mediator of fatigue in disease. Journal of
Neuroinflammation. 2017, 14:16
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