Products from BPS Bioscience require a minimum order value above 400€
Application: This kit is useful for cytokine detection in cell culture medium.
Description: The cytokine protein Interleukin-2 is an important factor for the maintenance of CD4+ regulatory T cells. It plays a critical role in the differentiation of CD4+ T cells into a variety of subsets as well as promotes CD8+ T-cell and NK cell cytotoxicity. The Colorimetric Human IL-2 Detection Kit is designed for detecting and quantifying human interleukin-2 in cell culture medium. Only a few simple steps on a microtiter plate are required for the assay. First, the capture antibody is coated on a 96-well plate. Next, samples containing IL-2 are incubated on the coated plate followed by detecting the captured IL-2 with the detection antibody. Finally, the plate is treated with streptavidin-HRP followed by addition of a colorimetric HRP substrate to produce color, which can then be measured using a UV/Vis spectrophotometer microplate reader.
Storage Stability: One year from date of receipt when stored as directed.
Supplied As: This kit comes in a convenient 96-well format, with capture and detection antibodies for IL-2, streptavidin-labeled HRP, blocking buffer, IL-2 standard, and colorimetric HRP substrate for a 96- well plate.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: Jiang, T., et al. Role of IL-2 in Cancer Immunotherapy. OncoImmunology, 2016. 5(6): e1163462
English
Deutsch
