IL-2 Luciferase Reporter Jurkat Cell Line

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Applications: The IL-2-luciferase Jurkat reporter cell line is suitable for monitoring the transcription activity of IL-2 in response to stimulants, and establishing cell-based screens for inhibitors that target specific IL-2 stimulating molecules.

Background: Interleukin-2 (IL-2) is a key cytokine important for proliferation and differentiation of T cells into effector T cells through interaction with the IL-2 receptor.

Description: Human IL-2 reporter construct is stably integrated into the genome of Jurkat T-cells. The firefly luciferase gene is controlled by a human IL-2 promoter.

Format: Each vial contains ~2 x 10^6 cells in 1 ml of 10% DMSO in FBS.

Host Cell Line: Jurkat

Mycoplasma Testing: This cell line has been screened using the MycoAlert™ Mycoplasma Detection Kit (Cat. No. LT07-118) to confirm the absence of Mycoplasma contamination. MycoAlert Assay Control Set (Cat. No. LT07-518) was used as a positive control.

Storage Stability: Store in liquid nitrogen immediately upon receipt.

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: BSL-1

References: 1.Weaver JR et.al. (2007) Characterization of the sequence and architectural constraints of the regulatory and core regions of the human interleukin-2 promoter. Mol. Immunol. 44: 2813-2819.2.Hughes CCW and Pober JS (1996) Transcriptional Regulation of the Interleukin-2 Gene in Normal Human Peripheral Blood T Cells. J. Biol. Chem. 271: 5369-5377.

• SKU: BPS60481
• Manufacturer: BPS Bioscience
• Manufacturer SKU: 60481
• Package Unit: 2 vials
• Quantity Unit: PAK
• Host: Human