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Applications: Great for screening small molecules and antibodies that inhibit the binding of IL-17A to IL-17RA.
Background: IL-23A is a pro-inflammatory cytokine that plays a key role in inflammation, autoimmunity, and host defense. Due to its role as a mediator of inflammation, the IL-23 pathway has become a popular target for treating diseases that have a strong inflammatory component such as bowel disease and colon cancer.
Description: The IL-23RA:IL-23A[Biotin] Inhibitor Screening Assay Kit is designed for screening inhibitors of IL-23A:IL-23RA interaction. The key to this kit is the high sensitivity of detection of biotin-labeled IL-23A by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, IL-23RA is coated on a 96-well plate. Next, IL-23A[Biotin] is incubated with IL-23RA on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can then be measured using a chemiluminescence reader.
Storage Stability: One year from date of receipt when stored as directed.
Supplied As: The IL-23RA-IL-23A[Biotin] Binding Assay Kit comes in a convenient 96-well format, with biotin-labeled IL-23A, purified IL-23 Receptor alpha (IL-23RA), streptavidin-labeled HRP, and assay buffer for 100 binding reactions.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. McKenzie, B.S., et al. 2006. "Understanding the IL-23-IL-17 immune pathway." Trends in Immunology 27(1): 17-23.
2. Iwakura, Y., and Harumichi I. 2006. "The IL-23/IL-17 axis in inflammation." The Journal of clinical investigation 116(5): 1218-1222.
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