Clone Name: PC61.5
Light Chain: Lambda
Product Description: The PC61.5 antibody is specific for mouse CD25, a 55 kDa surface protein also known as the Interleukin-2 Receptor alpha chain, or IL-2R alpha. CD25 may bind IL-2 by itself, although with low affinity and without induction of cell signaling. CD25 is also expressed within a high-affinity complex, along with the IL-2R beta chain (CD122) and the common gamma chain (CD132), to form a signaling receptor complex. Expression of CD25 varies during developmental stages of T and B cells, is induced on activated mature T and B cells, and is present on subsets of dendritic cells. CD25 signaling as part of the IL-2 receptor complex triggers T cell activation and proliferation, as well as modulating the differentiation and function of Th17 cells, T regulatory (Treg) cells, and dendritic cells. The PC61.5 antibody is used as a marker for T cells, B cells and dendritic cell subsets. Expression of CD25, CD4 and the transcription factor Foxp3 is regarded as a phenotypic signature for Treg cells. As such, this antibody is widely used to distinguish Treg cells from naïve or conventional T cells which are CD25. This clone has also been reported for depletion of Treg cells in vivo.
Application Note: *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Form: Liquid
Buffer (with preservative): 10mM NaH₂PO₄, 150mM NaCl, 0.09% Sodium azide.
Concentration: 0.5 mg/ml (Please refer to the vial label for the specific concentration.)
Uniprot ID: P01590/Q544I2
Purification: Purified by affinity chromatography
Conjugation: Unconjugated
Full Name: interleukin 2 receptor, alpha chain
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