Vector® NovaRED™ and ImmPACT™ NovaRED™ HRP substrates produce red reaction products. Unlike AEC, sections stained with either substrate should be dehydrated, cleared, and permanently mounted. Both chromogens are useful as alternatives to DAB or as a second color for multiple antigen labeling. Vector® NovaRED™ and ImmPACT™ NovaRED™ substrates also provide excellent color contrast in pigmented tissue such as melanoma. With the aid of imaging systems and software, the spectral profile of both substrates can be distinguished from other enzyme substrates in applications where antigens are co-localized. Sections stained with either substrate also can be viewed by darkfield microscopy. Vector® NovaRED™ and ImmPACT™ NovaRED™ substrates can be used for both manual and automated staining methods.