BioTek's Scratch Assay Starter Kit contains all the components needed to perform automated kinetic cell migration and wound healing assays in conjunction with the BioTek Lionheart™ Microscope or Cytation™ Cell Imaging Multi-Mode Reader.
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BioTek's AutoScratch™ Wound Making Tool for automatically creating repeatable scratches in the cell lawn
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The Scratch Assay App software calculates key statistics such as wound width,% confluence, and maximum wound healing rate.
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Suitable for 24- and 96-well microtiter plates (Corning)
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All reagents for making "scratches" are included in the kit.
Easy installation of the Scratch Assay App*
The special software supports automatic recording and evaluation of images. Various parameters, e.g. plate type, image capture and processing are already pre-programmed and can be customized. Simple export functions allow the presentation of results in MSExcel®, as well as the creation of videos and graphs.
- Gen5ImagePlus or Gen5ImagePrime is required for the installation
![](https://www.szabo-scandic.com/media/content/news/scratch_assay_starter_kit_biotek/csm_assayapp_95d3814feb.png)
REPRODUCIBLE WOUNDS
Manual scratch methods are very prone to error and poorly reproducible. BioTek's AutoScratch Woundmaker automates this process for 24- and 96-well plates, ensuring high-quality, consistent cuts across the entire cell culture plate.
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Predefined measurement protocols
You can start with a simple, predefined protocol. Various settings, e.g. temperature, assay runtime, kinetic interval, and other acquisition parameters are easily modifiable by simple navigation in the software for each experiment.
![](https://www.szabo-scandic.com/media/content/news/scratch_assay_starter_kit_biotek/messprotokolle.png)
Automatic calculations
When the images are taken, the Scratch Assay app automatically calculates the percentage cell confluence, the wound width, and the maximum wound healing rate (max V).
![](https://www.szabo-scandic.com/media/content/news/scratch_assay_starter_kit_biotek/csm_berechnungen_b352d29a62.png)
Automatic, multi-stage cleaning of the scratch pins
Built-in reagent tubes perform an automatic cleaning of the scratch pins and prevent carryover. The necessary cleaning reagents are included in the kit.
![](https://www.szabo-scandic.com/media/content/news/scratch_assay_starter_kit_biotek/csm_reinigung_456b2461b9.png)
AUTOMATED SCRATCH WOUND ASSAY WASH STEP USING MULTIFLO FX MULTI-MODE DISPENSER
Further software apps are in development. Already available ist he "Quantitative Assay App". Common assays are already pre-programmed: BCA Protein Assay App, Bradford Assay App, Lowry Assay App, and soon an app for the fluorometric quantification of nucleic acids.