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Encompassing Amino Acids: 186-530
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Assay was done in Kinase buffer containing 1 mM DTT using MBP substrate (0.2 mg/ml) and 10 mM ATP. Reaction was done at 30°C for 45 min. Amount of ATP transferred was calculated using Kinase-Glo reagent
Description: Human interleukin-1 receptor-associated kinase 1 (IRAK1), GenBank Accession No. NM_001569, a.a 186-530, with N-terminal His-tag, MW = 40 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 3 mM DTT, variable amounts of imidazole, and 20% glycerol.
Genbank: NM_001569
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C. Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 - 0.5%)
Tags: N-terminal His-tag
Uniprot: P51617
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Ku C.L. et al., J. Exp. Med. 2007; 204(10): 2407-2422.2. Hoarau C. et al., J. Immunol. 2007; 179(7): 4754-4765.
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