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Encompassing Amino Acids: 532-1132
Description: Human Jak2 containing the catalytic and the pseudo kinase domain (a.a. 532-1132), also known as Janus kinase-2, Just Another Kinase 2, Jak-2-JH1, JH2, GenBank Accession No. NM_004972, with N-terminal GST-Tag and C-terminal Avi-TagTM co-expressed with PTP1B in HEK293 expression system. This protein is enzymatically Biotin-labeled using AviTag™ technology. Percentage of phosphorylation ≤ 10%. MW=100 kDa.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20 mM Imidazole and 20% glycerol.
Genbank: NM_004972
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-Tag, C-terminal Avi-Tag
Uniprot: O60674
Warnings: Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended. If necessary, use carrier protein (BSA 0.1 - 0.5%).
Biosafety Level: Not applicable (BSL-1)
References: 1. Tono, C., et al., Leukemia. 2005; 19(10): 1843-1844.2. Goerttler, P.S., et al., Blood. 2005; 106(8): 2862-2864.
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