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Encompassing Amino Acids: 513-1132 (end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Concentration: 0.2 mg/ml
Description: Human Jak2 with V617F mutation, GenBank Accession No. NM_004972, a.a. 513-1132 (end), with N-terminal GST tag, C-terminal Avi-Tag, expressed in a HEK293 cell expression system and enzymatically Biotin-labeled using AviTag™ technology.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20mM GSH and 20% glycerol.
Genbank: NM_004972
Storage Stability: At least 6 months at -80°C. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 - 0.5%).
Tags: h N-terminal GST tag, C-terminal Avi-Tag
Uniprot: O60674
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Tono, C., et al., Leukemia. 2005; 19(10): 1843-1844.
2. Goerttler, P.S., et al., Blood. 2005; 106(8): 2862-2864.
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