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Encompassing Amino Acids: 536-812
Description: Human Jak2 with W659A, W777A, and F794H mutations, GenBank Accession No. NM_004972, a.a. 536-812, with Cterminal His-Avi-Tag, expressed in an Sf9 cell expression system and enzymatically Biotin-labeled using AviTag™ technology. MW=35 kDa.
Format: Aqueous buffer solution.
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween20, 140 mM Imidazole and 20% glycerol.
Genbank: NM_004972
Storage Stability: At least 6 months at -80°C.
Tags: C-Terminal His-Avi-Tag
Uniprot: O60674
Warnings: Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended. If necessary, use carrier protein (BSA 0.1 - 0.5%).
Biosafety Level: Not applicable (BSL-1)
References: 1. Tono, C., et al., Leukemia. 2005; 19(10): 1843-1844.2. Goerttler, P.S., et al., Blood. 2005; 106(8): 2862-2864.