Products from BPS Bioscience require a minimum order value above 400€Encompassing Amino Acids: 1-1090
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: 10 µl reaction mix containing assay buffer with 20 mM HEPES (pH 7.4), 50 mM NaCl, 500 µM α-ketoglutarate, 25 µM iron, 2 mM ascorbic acid, 0.01% Tween-20, 0.5 µM biotinylated peptide substrate, and JARID1A (80 - 400 ng) added to the wells. Add antibody against demethylated K4 peptide. Incubate for 30 min, then Streptavidin-conjugated secondary antibody followed by Alpha Screening detection.
Description: Human JARID1A, also known as KDM5A and RBBP2, GenBank Accession #NM_001042603), a.a. 1-1090 with C-terminal FLAG-tag, MW= 125 kDa, expressed in Sf9 cells via a baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 80 µg/ml FLAG peptide
Genbank: NM_001042603
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: C-terminal FLAG-tag
Uniprot: P29375
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Roesch, A., et al., (2005). Mod Pathol. 18(9):1249-57.
2. Zhou, X., et al., (2010). Cancer Res. 70(10):4214-21
3. Van Zutven, LJ., et al., (2006). Genes Chromosomes Cancer 45(5):437-46.Application Reference(s):
A small molecule modulates Jumonji histone demethylase activity and selectively inhibits cancer growth (2013)
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