Products from BPS Bioscience require a minimum order value above 400€Encompassing Amino Acids: 2-1090
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Assay conditions: 10 µl reaction mix containing assay buffer with 20 mM HEPES (pH 7.4), 50 mM NaCl, 500 µM α-ketoglutarate, 25 µM iron, 2mM ascorbic acid, 0.01% Tween-20, 0.5 µM biotinylated peptide substrate, and JARID1A (80 - 400 ng) added to the wells. Add antibody against demethylated K4 peptide. Incubate for 30 min, then Streptavidin-conjugated secondary antibody followed by Alpha Screening® detection.
Description: Human JARID1A, also known as KDM5A and RBBP2, GenBank Accession No. NM_001042603, a.a. 2-1090 with N-terminal His-Avi-tags and C-terminal FLAG-tag, MW= 128 kDa, expressed in Sf9 cells via a baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 80 µg/ml FLAG peptide, and 20% glycerol.
Genbank: NM_001042603
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-Avi-tags, C-terminal FLAG-tag
Uniprot: P29375
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Roesch, A., et al. (2005). Mod Pathol. 18(9):1249-57.
2. Zhou X., et al. (2010). Cancer Res. 70(10):4214-21
3. van Zutven LJ, et al. (2006). Genes Chromosomes Cancer 45(5):437-46.Application Reference(s):
A small molecule modulates Jumonji histone demethylase activity and selectively inhibits cancer growth (2013)
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