Products from BPS Bioscience require a minimum order value above 400€
Applications: Great for studying enzyme kinetics and HTS applications.
Contraindications: DMSO >1%, strong acids or bases, ionic detergents, high salt
Description: The JARID1B Chemiluminescent Assay Kit is designed to measure JARID1Bactivity for screening and profiling applications. JARID1B is a member of the Jumonji, AT-richinteractive domain 1 (JARID1) histone demethylase protein family. It binds directly, with severalother proteins, to retinoblastoma protein which regulates cell proliferation. The key to theJARID1B Chemiluminescent Assay Kit is a highly specific antibody that recognizes demethylatedsubstrate. With this kit, only three simple steps on a microtiter plate are required for detection ofdemethylase activity. First, JARID1B enzyme is incubated with the methylated H3 peptide for onehour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondaryantibody followed by the addition of the HRP substrate to produce chemiluminescence that can bemeasured using a chemiluminescence reader.
Storage Stability: One year from date of receipt when stored as directed.
Supplied As: The JARID1B Chemiluminescent Assay Kit comes in a convenient format, with a 96-well strip plate pre-coated with methylated histone H3 peptide substrate, primary antibody, secondary HRP-labeled antibody, demethylase assay buffer, and purified JARID1B.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: Klose, R.J., et al. Cell 2007; 128: 889.
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