Products from BPS Bioscience require a minimum order value above 400€
Applications: Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Background: JARID1B, also known as PLU-1 and KDM5B, is a JumonjiC(JmjC) and ARID domain-containing histone lysine demethylase that exhibits demethylationactivity toward di- and trimethyl-lysine 4 (H3K4me2/3) on histone H3.
Contraindications: Green and blue dyes that absorb light in the AlphaScreen® signalemission range (520-620 nm), such as Trypan Blue. Avoid the use of the potent singlet oxygenquenchers such as sodium azide (NaN3) or metal ions (Fe2+, Fe3+, Cu2+, Zn2+ and Ni2+). Thepresence of culture medium RPMI 1640 at >1% leads to signal reduction due to the presence of excess biotin and iron in this medium. MEM, which lacks these components, does not affectAlphaScreen® assays.
Description: The JARID1B Homogeneous Assay Kit is designed to measure JARID1B activity for screening and profiling applications. JARID1B, also known as PLU-1 and KDM5B, is a JumonjiC (JmjC) and ARID domain-containing histone lysine demethylase that exhibits demethylation activity toward H3-K4Me2 and H3-K4Me3. The JARID1B Homogeneous Assay Kit comes in a convenient AlphaLISA® format (Scheme 1), with biotinylated histone H3 peptide substrate, primary antibody, demethylase assay buffer, and purified JARID1B for 96 or 384 enzyme reactions. The key to the JARID1B Homogeneous Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing JARID1B enzyme is incubated with the biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts. Our histone demethylase assays utilize highly specific antibodies that recognize demethylated products. First, a sample containing the enzyme is incubated with a biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.
Storage Stability: At least 6 months at -80°C.
Supplied As: The JARID1B Homogeneous Assay Kit comes in a convenient AlphaLISA® format, with biotinylated histone H3 peptide substrate, primary antibody, demethylase assay buffer, and purified JARID1B for 96 or 384 enzyme reactions.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: Lahoud, M.H., et al. Genome Res 11 (8): 1327-34.
English
Deutsch
