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Applications: Great for screening small molecular inhibitors for drug discovery and HTS applications.
Background: JARID1B, also known as PLU-1 and KDM5B, is a JumonjiC (JmjC) and ARID domain-containing histone lysine demethylase that exhibits demethylation activity toward di- and trimethyl-lysine 4 (H3K4me2/3) on histone H3.
Description: The JARID1B TR-FRET Assay Kit is designed to measure activity of JARID1Bin a homogeneous 384 reaction format. This FRET-basedassay requires no time-consuming washing steps, making it especially suitable for highthroughput screening applications. The JARID1B TR-FRET Assay Kit comes in a convenientformat, with histone H3 peptide substrate, a Eu-labeled antibody against methylated K4 residueof Histone H3, demethylase assay buffer, TR-FRET detection buffer, dye-labeled acceptor, andpurified JARID1B for 384 enzyme reactions. The key to the JARID1B Assay Kit is a highlyspecific antibody that recognizes demethylated substrate. With this kit, only three simple stepson a microtiter plate are required for demethylase activity detection. First, a sample containingJARID1B enzyme is incubated with the biotinylated substrate. Next, antibody is added. Finally,dye-labeled acceptor is added followed by fluorescence reading.
Storage Stability: At least 6 months from date of receipt when stored as directed.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: Lahoud, M.H., et al. Genome Res. 11 (8): 1327-34.
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