Products from BPS Bioscience require a minimum order value above 400€
Applications: Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Background: JMJD2C is a JmjC-domain protein that exhibits demethylation activity toward H3-K9Me3 and H3-K36Me3.
Contraindications: DMSO >1%, strong acids or bases, ionic detergents, high salt
Description: The JMJD2C Chemiluminescent Assay Kit is designed to measure activity of the JMJD2C for screening and profiling applications. The key to the JMJD2C Chemiluminescent Assay Kit is a highly specific antibody thatrecognizes demethylated substrate. With this kit, only three simple steps on a microtiterplate are required for methyltransferase detection. First, a sample containing JMJD2Cenzyme is incubated with a sample containing assay buffer for one hour. Next, primaryantibody is added. Finally, the plate is treated with an HRP-labeled secondary antibodyfollowed by addition of the HRP substrate to produce chemiluminescence that can then bemeasured using a chemiluminescence reader.
Storage Stability: 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Supplied As: Kit comes in a convenient format, with a 96-well plate* precoated with the methylated histone H3 peptide substrate, primary antibody, the secondary HRP-labeled antibody, demethylase assay buffer, and purified JMJD2C for 100 enzyme reactions.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: Whetstine JR et al. (2006) Cell 125: 467.
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