Products from BPS Bioscience require a minimum order value above 400€
Applications: Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Background: JMJD2D is a JmjC-domain protein that exhibits demethylation activity toward H3-K9Me3 and H3-K9Me2.
Contraindications: DMSO >1%, strong acids or bases, ionic detergents, high salt
Description: The JMJD2D Chemiluminescent Kit is designed to measure activity of JMJD2D for screening and profiling applications. The key tothe JMJD2D Chemiluminescent Assay Kit is a highly specific antibody that recognizesdemethylated substrate. With this kit, only three simple steps on a microtiter plate are required formethyltransferase detection. First, a sample containing JMJD2D enzyme is incubated with asample containing assay buffer for one hour. Next, primary antibody is added. Finally, the plate istreated with an HRP-labeled secondary antibody followed by addition of the HRP substrate toproduce chemiluminescence that can then be measured using a chemiluminescence reader.
Storage Stability: 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Supplied As: Kit comes in a convenient format, with 8-well strips pre-coated with the methylated histone H3 peptide substrate, primary antibody, the secondary HRP-labeled antibody, demethylase assay buffer, and purified JMJD2D for 100 enzyme reactions.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: Whetstine, J.R. et al., Cell 2006; 125: 467.
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