Products from BPS Bioscience require a minimum order value above 400€Encompassing Amino Acids: 2-354
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: 10 µl reaction mix containing assay buffer with 20 mM HEPES (pH 7.4), 50 mM NaCl, 1 mM TCEP, 500 µM α-ketoglutarate, 25 µM iron, 2 mM ascorbic acid, 0.01% Tween-20, 0.5 µM biotinylated peptide substrate, and JMJD2D (5 - 25 ng) added to the wells. Add antibody against demethylated K9 peptide. Incubate for 30 min, then Streptavidin-conjugated secondary antibody followed by Alpha Screening detection.
Description: Human JMJD2D (GenBank Accession No. NM_018039), amino acids 2-354, with N-terminal GST tag, MW=67 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris pH 8.0, 110mM NaCl, 2.2 mM KCl, 3 mM DTT and20% glycerol
Genbank: NM_018039
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q6B0I6
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Katoh, M., Int J Oncol. 2004 Jun; 24(6):1623-8.
2. Shin, S., et al., Biochem Biophys Res Commun. 2007 Feb 23; 353(4):973-7.Application Reference(s):
A small molecule modulates Jumonji histone demethylase activity and selectively inhibits cancer growth (2013)
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