Products from BPS Bioscience require a minimum order value above 400€Encompassing Amino Acids: 2-337
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: 10 µl reaction mix containing assay buffer with 20 mM HEPES (pH 7.4), 150 mM NaCl, 500 µM α-ketoglutarate, 25 µM iron, 2 mM ascorbic acid, 0.01% Tween-20, 0.5 µM biotinylated peptide substrate, and JMJD2E (100 - 400 ng) added to the wells. Add antibody against demethylated K9 peptide. Incubate for 30 min, then Streptavidin-conjugated secondary antibody followed by Alpha Screening detection.
Description: Human JMJDE , also known as KDM4DL (GenBank Accession No. NM_001161630), a.a. 2-337 with N-terminal GST-tag, MW=65 kDa, expressed in Sf9 cells using a Baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathione
Genbank: NM_001161630
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: B2RXH2
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Fodor, B.D., et al., (2006). Genes Dev. 20(12): 1557-1562.
2. Beyer, S., et al., (2008). J. Biol. Chem. 283(52):36542-52.Application Reference(s):
A small molecule modulates Jumonji histone demethylase activity and selectively inhibits cancer growth (2013)
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