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Encompassing Amino Acids: full length
Applications: Useful as a substrate for kinase assays.
Description: Human JNK1-β1(K55M), also known as MAPK8 (K55M) (GenBank Accession No. NM_139046), full length with mutation of lysine55 to methionine, and N-terminal GST-tag, MW=~70 kDa, expressed in E. coli. The K55M mutation prevents ATP binding, making the kinase inactive.
Format: Aqueous buffer solution
Formulation: 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 10% glycerol, and 3 mM DTT.
Genbank: NM_139046
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: P45983
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Derijard, B. et al., Cell. 76(6):1025-37 (1994).
2. Kolodziejczyk, S M. et al., Curr. Biol. 11(16):1278-82 (2001).
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