The Kinetic-QCL Kinetic Chromogenic LAL Assay is a quantitative, kinetic assay for the detection of Gram-negative bacterial endotoxin. A sample is mixed with the reconstituted LAL reagent in a 96-well plate and placed in an incubating plate reader that measures absorbance at 405 nm. The reaction is automatically monitored over time for the appearance of a yellow color.
In the presence of endotoxin, the lysate will begin to cleave the chromogenic substrate, causing the solution to become yellow. The time required for the change is inversely proportional to the amount of endotoxin present. The concentration of unknown samples can then be calculated from a standard curve. It is the chromogenic nature of the Kinetic-QCL Kinetic Chromogenic LAL Assay that makes it the most appropriate choice for testing small volume parenterals, vaccines, antibiotics and biologicals. The Kinetic-QCL Assay is less impacted by inhibitory products which may interfere with the clotting mechanism in turbidimetric and gel clot assays. With a sensitivity range of 0.005 EU/ml - 50.0 EU/ml, the Kinetic-QCL Kinetic Chromogenic LAL Assay is the most sensitive of the LAL assays.
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