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Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: KMO #11307 was tested with various amounts of enzyme. The reactions in KMO Assay Buffer (50 mM Sodium Phosphate, pH 7.5, 0.1% (w/v) Brij-35) contains 200 µM NADPH, 400 µM L-Kynurenine, and various concentrations of KMO. The reaction mixture incubated for 90 minutes while reading the UV absorption signal. For the negative control (blank), assay buffer was added instead of the enzyme. Absorption signals at 340 nm were measured using a Tecan Infinite M1000 plate reader.
Description: Recombinant human KMO (kynurenine 3-monooxygenase), full length, encompassing amino acids 1-486(end). This construct contains a C-terminal His-Flag-tag (6xHis). The recombinant protein was affinity purified. EC 1.14.13.9
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 100 µg/ml FLAG peptide, 0.2 mM TCEP and 20% glycerol.
Genbank: NM_003679
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Supplied As: Aqueous buffer solution.
Tags: C-terminal His-FLAG-tags
Uniprot: O15229
Warnings: Avoid multiple freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Zwilling, D., et. al., Cell. 2011; 6(10):863-874.
2. Giorgini, G., et al., Nature Genetics. 2005; 37: 526-531.
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